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To avoid external contamination, the lab users wore cotton lab coats and clothing, regularly cleaned the chamber with cleanroom wipes and ethanol after each sample and stored each sample in a glass container. The glass file was sanitized with ethanol after every test to preclude contamination. Power input was calculated using the value of torque measured (Datum electronics, M425, Isle of Wight, UK).

This is because it contains organisms that can cause illness or infection, for example shigella. This is a bacterial infection of the intestine that causes severe diarrhoea and is often mistaken for food poisoning. It can be caught during oral-anal sex and giving oral sex after anal sex&nbsp